Northern Virginia vs. the rest of the state. People in Southside (south-central Virginia) and the Shenandoah Valley can't stand many of the people who come out of Fairfax and Arlington County, whom they often believe act condescending to them. The fact that these areas, as well as Loudoun and Prince William counties, are trending towards liberalism doesn't help. Meanwhile many Northern Virginians (mostly transplants from other states) can't stand the rural areas and the small towns and think that NoVa should secede from the rest of Virginia.
As for regional Virginia rivalries, you've got Lynchburg vs. Roanoke and metro Richmond vs. Chesterfield County (this one is mainly based on politics).
The Tidewater vs the Piedmont.
And everyone who has a sane mind hates Princess Anne.
